Optical Tweezers for Biology

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Optical tweezers are used to finely capture and move microorganisms or anything microscopic. For this experiment, the focus was to capture biological specimens, and measure the amount of energy they have. With that it can be determine how much energy is produced in order for them to escape a laser trap.

The first thing that was done was the set up capturing apparatus. To start this the first things that should be done is preparing the fiber optic laser that will be used. There should be equipment in the room to allow for practice before the real one should be done.

Following up with the apparatus set up is the laser set up

After the fiber optic is set up to be a laser, finish setting up the apparatus

There is a solution known as methyl cellulose. It has the ability to hinder and slow the movement of any microorganism as well as other microscopic mass present. This is the way that we bypassed the problem of the microscopic masses moving pass our field of view. They move extremely quickly and any twitch to focus or lock on to the target would shake it around making it even harder to capture.

The first thing to try to capture are glass beads. The glass beads are good practice as they have a clear path and direction that they are traveling in. They don't have a tail and will therefore move in a easily predictable path. There are two types of beads to pick from. There is a vial that had PEG and one that does not contain PEG. PEG is a short name for Polyethylene glycol. It is meant to prevent the beads from being trapped against the glass surface of the coverslip or the slide. The PEG is better for starting to practice and capture the beads. The ones without the PEG is great for fine tuning the apparatus to focus in more on the beads.

Once you have become good enough at capturing beads, the next step is to catch biological organism. This follows the same process as the beads.